VTM solution for virus inactivation and lysis

 

In addition to the lysing components, the inactivated virus preservation solution usually contains detergents that denature proteins and destroy the membrane structure, biological buffers, inhibitors that prevent nucleases from degrading nucleic acids, and reagents that maintain the stability of the nucleic acid structure. The lysis system may also add protease to cut the protein into s-mall fragments to promote the separation of protein and nucleic acid.

At present, there are many known methods for lysing viruses. Two of the more common methods are heating method and concentrated salt method:

The concentrated salt method uses high concentration of salt to destroy the secondary bond between nucleic acid and protein, disassociating the nucleoprotein and releasing the viral nucleic acid. Usually, guanidine hydroch-loride, guanidine isothiocyanate or non-guanidine salt cleavage salt can be used to directly lyse and inactivate the virus without heating the sample, which is relatively simple.

The heating method is based on heating to a temperature value of 80 C ~ 100 C, because generally samples are frozen at -70 C, generally need to be heated for 5 to 10 minutes, this operation is relatively simple, only one step can be used for The template for nucleic acid PCR amplification does not require any operation steps such as centrifugation and extraction, and is faster than the commonly used CheleX100 extraction method and phenol/chloroform methods. Although this method is simple, the amount of nucleic acid extracted will be relatively s-mall.