Cellulose is an unbranched glucose polymer composed of anhydro-D-glucose units linked by 1, 4-b-D-glucoside bonds. These glycosidic bonds can be hydrolysed by cellulolytic enzymes. The native structure of the cellulose is composed of crystalline and less organized amorphous regions. SUKACell-N5000 is an enzyme preparation obtained from the submerged fermentation of a high cellulase-producing microbial strain. It is widely used in textile and garment stone wash industries.

The 1, 4-b-D-glucosidic linkages in cellulose, lichenin and cereal b-D glucans are hydrolyzed to release free glucose units through the successive action of cellulase enzymes.

The less organized amorphous region at the center of the cellulose chain is initially attacked by endoglucanase by random cutting of the b-1, 4 glycosidic linkages within the chain thereby producing cello-oligosaccharides. Cellobiohydrolases I and II act on the cello-oligosaccharides from the reducing end and non-reducing end of the cello-oligosaccharides to release cellobiose. b- glucosidase act on the released cellobiose to produce b–D glucose units.

SUKACell-N5000 will function from a pH of 5.5 to 7.5, better perform at 6.0 to 6.5. It will function from 40℃ to 60℃，and the optimum temperature is 55℃ 。 

Appearance: Tan powder (Note that color does not affect or reflect activity.)

Odor: Slight fermentation odor

pH (1% soln)：3.5-5.5；

SUKACell-N5000( activity is 5000 U/g)。

High activity product is available upon request of our customers.

Reducing sugar method: At a pH of 6. 0 and temperature of 50 ℃, to produce the amount of reducing sugar equivalent to 1 mg glucose, it needs l g solid enzyme (or 1mL liquid enzyme) and 1 h hydrolyze sodium carboxymethyl cellulose substrate, as 1 enzyme activity unit, state as u/g (or u/mL). CMCA – DNS for short.

Light industry standard of the People's Republic of China, QB 2583 2003

Ø        SUKACell-N5000 - is a kind of netural solid cellulase, used for biofinishing, significantly soften 100% cotton fabrics, both woven and knits.

Ø        SUKACell-N5000 – provide soft touch except used for jean leaven dye processing.

Ø        SUKACell-N5000 - can obviously improve clothing surface magnitude.

1. Desize thoroughly.

2. Drain liquor then fill machine to liquor ratio of 1:10. (jean/ water)

3. Add pumice 0-0.5 kg, heat bath to 40℃-60℃, and the optimum temperature is 55℃ .

4. Add SUKACell –N5000 at the rate of 0.25-0.75% owg.

 

5. Adjust pH to 5.5-7.5 with pH 7.0 as an optimum.

6. Process for 30-50 minutes depending on desired effect.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                

7. Add water and soften after drainage.

NOTE: Under the temperature of 80℃ and with the PH10.0, the enzyme will be deactivation. Some denim fabrics of lower quality may have severe backstaining problems that can be overcome with our ES resist.

Use soda ash 1-3 grams and resist ES 1-2 grams (water ratio) to wash out ferment remaining residue, after that you can adopt our consistent lubricant/ film/ organic silicon oil.

The information presented is believed to be accurate. However, said information and products are offered without warranty or guarantee. Sales commitment should pay legal responsibility. We recommend that the prospective user determine the suitability of our materials and suggestions before adopting them on a commercial scale.

SUKACell-N5000 is packaged in 25 kg plastic drum for the solid form.

Alternative packaging is available upon request for smaller or larger volumes. 

 Store in a cool, dry place and away from direct sunlight. SUKACell-N5000 maintains its activity for considerably longer time when stored at lower temperatures (below 25℃). Do not let freeze.

SUKACell-N5000 is non-toxic and biodegradable. However, unnecessary contact with the product should be avoided. Long term exposure to protein based products such as SUKACell-N5000 may sensitize certain individuals to the product. Wash hands with warm soapy water after handling. Keep out of reach of children.

SUKAHAN (Weifang) Bio-Technology Co., Ltd will assist customers with the use of our products in applications development.